This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Homology
The homologs of KRAS is obtained using Homologene[1]. Up to 11 homologs of KRAS was found, of which 5 is included here.. For ease of comparison, the human KRAS gene will be listed first before the other homologs.
Homo sapiens (Humans)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)
Gene accession number:NC_000012.11
Protein accession number: NP_004976.2
Pan troglodytes (Chimpanzee)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_006479.2
Protein accession number: NP_004976.2
Mus musculus (Mouse)
Gene name: Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_000072.5
Protein accession number: NP_067259.3
Bos taurus (Cow)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_007303.4
Protein accession number: NP_001103471.1
Canis lupus familiaris (Dog)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_006600.2
Protein accession number: XP_540523.2
Caenorhabditis elegans (Roundworm)
Gene name: let-60 LEThal
Gene accession number: NC_003282.6
Protein accession number: NP_502213.3
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS)
Gene accession number:NC_000012.11
Protein accession number: NP_004976.2
Pan troglodytes (Chimpanzee)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_006479.2
Protein accession number: NP_004976.2
Mus musculus (Mouse)
Gene name: Kras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_000072.5
Protein accession number: NP_067259.3
Bos taurus (Cow)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_007303.4
Protein accession number: NP_001103471.1
Canis lupus familiaris (Dog)
Gene name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene accession number: NC_006600.2
Protein accession number: XP_540523.2
Caenorhabditis elegans (Roundworm)
Gene name: let-60 LEThal
Gene accession number: NC_003282.6
Protein accession number: NP_502213.3
FASTA text file of Protein homologs
| fasta-_protein.txt |
Alignment
This alignment analysis of the proteins of the homologs was obtained using T-coffee [2]
| alignment.pdf |
The above figure was obtained from TreeFam.
Boxes correspond to Pfam domains, while lines to non-domain regions. Dark lines or boxes show matched parts, while light ones gaps. Vertical dark blue lines indicate splicing boundaries. [3]
Boxes correspond to Pfam domains, while lines to non-domain regions. Dark lines or boxes show matched parts, while light ones gaps. Vertical dark blue lines indicate splicing boundaries. [3]
Analysis
The results using the protein sequences in the analysis using T-coffee varies greatly from the ones using the DNA FASTA sequences. In the DNA T-coffee alignment analysis, there is only bad and weak alignment among the genes, however in the protein analysis the results indicate strong homology. This is likely because the proteins of all the homologs are short enough so they do not need to be truncated. Thus, the conserved and fuctional parts of the protein is actually quite similar among all the species.
References
1) Homologene
http://www.ncbi.nlm.nih.gov/homologene
2) T-coffee
http://www.ebi.ac.uk/Tools/msa/tcoffee/
3) TreeFam
http://www.treefam.org
http://www.ncbi.nlm.nih.gov/homologene
2) T-coffee
http://www.ebi.ac.uk/Tools/msa/tcoffee/
3) TreeFam
http://www.treefam.org
